Gunson H H, Phillips P K, Stratton F
J Clin Pathol. 1974 May;27(5):356-8. doi: 10.1136/jcp.27.5.356.
Using the AutoAnalyzer, the percentage agglutination effected by the anti-D antisera studied showed a varied dependence on the ambient temperature over the manifold subsequent to the incubation period at 37 degrees C. This leads to assays which are a function of the ambient temperature. It is suggested that the entry of a relatively large volume of rouleaux-dispersing agent results in an elution of bound antibody to a new position of equilibrium, the shift being dependent on the particular equilibrium constant of the antibody and the rate of its attainment on the ambient temperature. A constant ambient temperature will lead to greater accuracy of anti-D assay.
使用自动分析仪，所研究的抗-D抗血清引起的凝集百分比在37℃温育期后的多种情况下显示出对环境温度的不同依赖性。这导致检测结果成为环境温度的函数。有人提出，加入相对大量的红细胞缗钱状形成分散剂会导致结合抗体洗脱至新的平衡位置，这种转变取决于抗体的特定平衡常数及其在环境温度下达到平衡的速率。恒定的环境温度将提高抗-D检测的准确性。
