使用自动分析仪进行抗-D定量检测时的操作误差和固有误差。
Manipulative and inherent errors in anti-D quantitation using the AutoAnalyzer.
作者信息
Gunson H H, Phillips P K, Stratton F
出版信息
J Clin Pathol. 1972 Mar;25(3):198-205. doi: 10.1136/jcp.25.3.198.
Abstract
The limits of accuracy for the quantitation of anti-D using the AutoAnalyzer have been reduced from +/- 26% with manually prepared dilutions to +/- 14% using an automatic pipette, as expressed by the 95% confidence limits. The error inherent in the AutoAnalyzer was estimated to contribute +/- 10% to the overall error. Problems associated with the reproducibility of this method for anti-D quantitation have been investigated, namely, the effect of the age of a given test cell, the use of different test cells, loss of sensitivity of the machine over a given day, and the reproducibility of results obtained at different positions on the standard graph.
摘要
使用自动分析仪定量检测抗 - D时，其准确度的限度已从手动制备稀释液时的±26%降低至使用自动移液器时的±14%，以95%置信限度表示。据估计，自动分析仪固有的误差对总误差的贡献为±10%。已对该抗 - D定量方法的重现性相关问题进行了研究，即给定检测细胞的保存时间、不同检测细胞的使用、机器在给定日期内的灵敏度损失以及在标准曲线上不同位置获得的结果的重现性。
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Automation in the blood transfusion laboratory: I. Antibody dectection and quantitation in the technicon autoanalyzer.输血实验室中的自动化：I. 技术康自动分析仪中的抗体检测与定量分析
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