Ginzburg M, Ginzburg B Z
J Membr Biol. 1976 Mar 18;26(2-3):153-71. doi: 10.1007/BF01868871.
Changes in cell volume and ion content of a Halobacterium species are described in terms of the NaCl concentration (0.5--3.5M) and pH(4-8) of the suspending medium. Cell volume, per unit content of protein of bacteria in stationary phase cultures, rose as the [NaCl] of the growth medium was increased. Logarithmic-phase bacteria shrank as the pH fell from 7 to 5.5. These changes are characteristic of bacteria with a moderate or rapid rate of O2 consumption. Starving (i.e. nonmetabolizing) bacteria, on the other hand, did not change in size within the above ranges of [NaCl] and pH. At lower values, however, such bacteria swelled and eventually lysed. Effects of low pH on cell ions are compared in metabolizing and starving bacteria, and it is shown that changes in the state of the cell K are correlated with movements of cell Na. It appears that the cell K is used to maintain cell [Na] below the NaCl concentration of the medium. The results are explained in terms of a model involving interactions between polyelectrolytes, salts and water in the concentrated cytoplasm of these halophilic organisms.
根据悬浮介质的氯化钠浓度（0.5 - 3.5M）和pH值（4 - 8），描述了一种嗜盐菌的细胞体积和离子含量的变化。在稳定期培养物中，细菌单位蛋白质含量的细胞体积随着生长培养基中[NaCl]的增加而增大。对数期细菌随着pH值从7降至5.5而收缩。这些变化是耗氧速率适中或较快的细菌的特征。另一方面，饥饿（即不进行代谢）的细菌在上述[NaCl]和pH值范围内大小不变。然而，在较低值时，这类细菌会膨胀并最终裂解。比较了低pH值对代谢和饥饿细菌细胞离子的影响，结果表明细胞K状态的变化与细胞Na的移动相关。似乎细胞K用于将细胞[Na]维持在低于培养基NaCl浓度的水平。根据一个涉及这些嗜盐生物浓缩细胞质中聚电解质、盐和水之间相互作用的模型对结果进行了解释。
