Matheson A T, Sprott G D, McDonald I J, Tessier H
Can J Microbiol. 1976 Jun;22(6):780-6. doi: 10.1139/m76-114.
An unidentified halophile isolated from plates of a complex agar medium containing 4.25 M NaCl showed optimum growth in broths containing 0.5-1.0 M NaCl but exhibited a wide range of growth from 0.045-4.5 M. The organism can be classified as a facultative halophile with wide salt tolerance. Logarithmic phase cells grown in media containing 0.5 M NaCl were rod-shaped in long chains which changed to smaller, single, or paired cells in stationary growth. The internal Na+ and K+ concentrations were 0.05 M and 0.34 M for logarithmic phase cells and 0.29 and 0.32 M for stationary phase cells. In 4.3 M NaCl media the cells were rod-shaped throughout the growth cycle, occurring primarily in pairs. The internal Na+ K" concentrations in cells in logarithmic phase growth were 0.62 M and 0.58 M while in stationary phase growth these values were 1.01 M and 0.66 M respectively. In contrast, logarithmic phase cells of the extreme halophile Halobacterium cutirubrum had internal Na+ and K+ concentrations of 0.80 M and 5.32 M when grown in 3.3 M NaCl. The internal Na+ and K+ concentrations, therefore, in the unidentified halophile do not resemble those found in H. cutirubrum but are much closer to those present in Escherichia coli.
从含有4.25 M氯化钠的复合琼脂培养基平板上分离出的一种未鉴定嗜盐菌，在含有0.5 - 1.0 M氯化钠的肉汤中生长最佳，但在0.045 - 4.5 M的范围内都能生长。该生物体可归类为具有广泛耐盐性的兼性嗜盐菌。在含有0.5 M氯化钠的培养基中生长的对数期细胞呈长链状杆形，在稳定生长期会变成较小的单个或成对细胞。对数期细胞的内部钠离子和钾离子浓度分别为0.05 M和0.34 M，稳定期细胞为0.29 M和0.32 M。在4.3 M氯化钠培养基中，细胞在整个生长周期中均为杆形，主要成对出现。对数期生长的细胞内部钠离子和钾离子浓度分别为0.62 M和0.58 M，而在稳定期生长时，这些值分别为1.01 M和0.66 M。相比之下，极端嗜盐菌红皮盐杆菌在3.3 M氯化钠中生长时，对数期细胞的内部钠离子和钾离子浓度分别为0.80 M和5.32 M。因此，未鉴定嗜盐菌的内部钠离子和钾离子浓度与红皮盐杆菌不同，但更接近大肠杆菌中的浓度。
