Wesson L G, Colberg J E, deGuzman A, Elsasser W, Dunn S
Transplantation. 1979 Jun;27(6):380-3.
Hilar drainage fluid of dog kidneys was analyzed as an approximation to renal extracellular fluid after preservation by flushing with chilled high K-low Na solution (Collins C4) followed by ice-cold storage for 24 and 48 hr in a bath of flushing medium. Compared with the medium, Na and Cl were increased to 30 mM/liter and K decreased slightly to 93 mM/liter. Glucose decreased, whereas lactate, lactic dehydrogenase, and creatine phosphokinase increased by significant amounts in both the drainage fluid and bath. The inulin space of the undrained kidney average 37% of wet weight. Calculated intracellular Na and Cl concentrations averaged 50 and 37 mM/kg cell water while K remained within normal limits. A significant fraction of red blood cells retained during initial flushing entered the effluent during storage. Bath and effluent composition of a human cadaver kidney approximated those of a dog.
通过用冷的高钾低钠溶液（柯林斯C4）冲洗后保存，然后在冲洗介质浴中冷藏24和48小时，对犬肾的肾门引流液进行分析，作为肾细胞外液的近似值。与介质相比，钠和氯增加到30 mM/升，钾略有下降至93 mM/升。葡萄糖减少，而乳酸、乳酸脱氢酶和肌酸磷酸激酶在引流液和浴液中均显著增加。未引流肾脏的菊粉空间平均为湿重的37%。计算得出的细胞内钠和氯浓度平均为50和37 mM/kg细胞水，而钾保持在正常范围内。最初冲洗过程中保留的相当一部分红细胞在储存期间进入流出液。人尸体肾的浴液和流出液成分与犬肾近似。
