Mattimore V, Udupa K S, Berne G A, Battista J R
Department of Microbiology, Louisiana State University, Baton Rouge 70803, USA.
J Bacteriol. 1995 Sep;177(18):5232-7. doi: 10.1128/jb.177.18.5232-5237.1995.
Natural transformation was used to help define a collection of ionizing radiation-sensitive strains of Deinococcus radiodurans. Three putative rec mutations were identified, as were three pol alleles. Forty of the ionizing radiation-sensitive strains were placed into 16 linkage groups, and evidence obtained indicates that each linkage group consists of a cluster of mutations not more than 1,000 bp apart. In addition, a new class of D. radiodurans mutant was described that, although radioresistant, appears to recover from ionizing radiation-induced DNA damage slowly relative to other strains of D. radiodurans.
利用自然转化来帮助确定一组耐辐射球菌属的电离辐射敏感菌株。鉴定出了三个假定的rec突变以及三个pol等位基因。40个电离辐射敏感菌株被归入16个连锁群,所获得的证据表明每个连锁群由相距不超过1000 bp的一组突变组成。此外,还描述了一类新的耐辐射球菌属突变体,尽管它具有抗辐射能力,但相对于其他耐辐射球菌属菌株而言,它从电离辐射诱导的DNA损伤中恢复的速度似乎较慢。