维生素微生物测定法的改进。
Improvement of microbial assays of vitamins.
作者信息
Heed E J
出版信息
Appl Microbiol. 1972 Aug;24(2):286-7. doi: 10.1128/am.24.2.286-287.1972.
Abstract
A method for the improvement of microbial assays of vitamins, which involves the addition of a surfactant to the incubated test, was developed. This surfactant tends to eliminate bacterial clumping, giving a uniform suspension of single cells, thereby making the turbidity readings less erratic and the actual assay standard curves more closely related to the desired theoretical curve.
摘要
一种改进维生素微生物测定法的方法被开发出来，该方法涉及在培养后的测试中添加一种表面活性剂。这种表面活性剂倾向于消除细菌聚集，形成单细胞的均匀悬浮液，从而使浊度读数更稳定，实际测定标准曲线与理想理论曲线的相关性更强。
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本文引用的文献
1
Precise standardization of reagents for complement fixation.补体结合反应试剂的精确标准化
Am J Trop Med Hyg. 1963 Jan;12:103-16. doi: 10.4269/ajtmh.1963.12.103.
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[Fractionation of human blood plasma proteins].[人血浆蛋白的分级分离]
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Appl Microbiol. 1969 Dec;18(6):1088. doi: 10.1128/am.18.6.1088-1088.1969.
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Immunoprecipitation analysis of soluble complement-fixing antigens of dengue viruses.
J Immunol. 1970 Oct;105(4):838-45.
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