从牛跟腱中分离糖蛋白及其与胶原蛋白的相互作用。
The isolation of glycoproteins from bovine achilles tendon and their interaction with collagen.
作者信息
Anderson J C, Jackson D S
出版信息
Biochem J. 1972 Mar;127(1):179-86. doi: 10.1042/bj1270179.
Abstract
Two glycoprotein fractions, A and B, were isolated from bovine achilles tendon. Glycoprotein A was prepared from a 0.2m-sodium chloride extract and glycoprotein B was isolated from a 3m-magnesium chloride extract. They were free from serum proteins. Glycoprotein A was essentially free of collagen, but glycoprotein B contained about 8% collagen. Both glycoproteins gave several bands on isoelectric focusing. This technique was also used to demonstrate that both glycoprotein fractions interacted strongly with acid-soluble calf skin tropocollagen. It is concluded that these fractions are true components of tendon, and that they may have some function in the macromolecular organization of the tissue.
摘要
从牛跟腱中分离出了两种糖蛋白组分，A和B。糖蛋白A是从0.2m氯化钠提取物中制备的，糖蛋白B是从3m氯化镁提取物中分离出来的。它们不含血清蛋白。糖蛋白A基本不含胶原蛋白，但糖蛋白B含有约8%的胶原蛋白。两种糖蛋白在等电聚焦时都出现了几条带。该技术还用于证明两种糖蛋白组分都与酸溶性小牛皮原胶原蛋白强烈相互作用。得出的结论是，这些组分是肌腱的真正成分，并且它们可能在组织的大分子组织中具有某些功能。
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