Postacchini F, Accinni L, Natali P G, Ippolito E, DeMartino C
Cell Tissue Res. 1978 Dec 14;195(1):81-97. doi: 10.1007/BF00233678.
The regenerated tissue which fills the gap between the stumps of sectioned and unsutured rabbit calcaneal tendon was studied by immunofluorescence, light and electron microscopy from 2 days to 30 weeks after surgery. In the early stages, the newly formed tissue consisted of few connective tissue cells of variable shape dispersed in an abundant intercellular matrix. At 7 days after tenotomy most of the cells were spindle shaped and arranged along the major tendon axis. They showed a well developed rough endoplasmic reticulum, a prominent Golgi complex and bundles of thin and thick filaments. Moreover, they appeared intensely stained when treated with anti-actin and anti-myosin sera. The bulk of the intercellular matrix consisted of bundles of collagen fibers, mostly arranged parallel to the cells. In the subsequent stages the regenerating tissue became more compact, acquiring the morphological characteristics of tendon tissue. At 30 weeks after tenotomy, however, it did not show yet the typical texture of the normal adult tendon. The tenocytes were more numerous and less uniformly distributed, and contained a greater amount of ergastoplasm and contractile proteins. The collagen fibers were similar in size to those of the neonatal normal tendon and the elastic fibers appeared often immature.
通过免疫荧光、光学显微镜和电子显微镜，对切断但未缝合的兔跟腱残端之间在术后2天至30周填充间隙的再生组织进行了研究。在早期阶段，新形成的组织由少数形状各异的结缔组织细胞组成，这些细胞分散在丰富的细胞间基质中。跟腱切断术后7天，大多数细胞呈纺锤形，并沿肌腱主轴线排列。它们显示出发育良好的粗面内质网、突出的高尔基体复合体以及粗细肌丝束。此外，在用抗肌动蛋白和抗肌球蛋白血清处理时，它们呈现强烈染色。细胞间基质的大部分由胶原纤维束组成，大多与细胞平行排列。在随后的阶段，再生组织变得更加致密，获得了肌腱组织的形态特征。然而，在跟腱切断术后30周，它尚未表现出正常成年肌腱的典型结构。腱细胞数量更多且分布不那么均匀，并且含有更多的内质网和收缩蛋白。胶原纤维的大小与新生正常肌腱的相似，弹性纤维往往不成熟。
