Nieto M, Perkins H R, Reynolds P E
Biochem J. 1972 Jan;126(1):139-49. doi: 10.1042/bj1260139.
Vancomycin inhibited the growth of Bacillus megaterium, Staphylococcus aureus and Micrococcus lysodeikticus, and in cell-free preparations from B. megaterium it inhibited the formation of mucopeptide and enhanced the accumulation of the lipid intermediate in the biosynthetic pathway. All these inhibitory processes were reversed by the presence of a synthetic peptide analogous to un-cross-linked mucopeptide side chains, namely diacetyl-l-diaminobutyryl-d-alanyl-d-alanine. A considerable amount of vancomycin was found in recovering cells, whether recovery was caused by peptide or took place naturally because a low initial concentration of antibiotic was used. In cell-free preparations pretreated with vancomycin, continued inhibition of mucopeptide synthesis depended on the presence of cell-wall material. This inhibition was also reversible by added peptide.
万古霉素可抑制巨大芽孢杆菌、金黄色葡萄球菌和溶壁微球菌的生长，在巨大芽孢杆菌的无细胞制剂中，它可抑制粘肽的形成，并增强生物合成途径中脂质中间体的积累。所有这些抑制过程都可被一种类似于未交联粘肽侧链的合成肽（即二乙酰基-L-二氨基丁酰-D-丙氨酰-D-丙氨酸）逆转。在恢复生长的细胞中发现了大量万古霉素，无论恢复是由肽引起的，还是因为使用了低初始浓度抗生素而自然发生的。在用万古霉素预处理的无细胞制剂中，粘肽合成的持续抑制取决于细胞壁物质的存在。这种抑制也可被添加的肽逆转。
