Garen A, Gehring W
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2982-5. doi: 10.1073/pnas.69.10.2982.
The eggs produced by deep orange females (dor/dor) of Drosophila melanogaster have a defect that causes early death of the deep orange embryos derived from these eggs. Ovaries transplanted from deep orange females to normal female hosts continue to produce defective eggs, showing that the dor mutation directly affects an ovarian function. In heterozygous embryos (dor(+)/dor) derived from eggs of deep orange females, the presence of a paternal dor(+) gene enables normal development to occur in about half of the embryos. Thus, the egg defect caused by the dor mutation is repairable after fertilization by the action of a dor(+) gene. The deep orange egg defect has also been repaired by injection of cytoplasm from unfertilized normal eggs into deep orange embryos at the syncitial preblastoderm stage of development. About one-third of the injected deep orange embryos were able to continue their development to an advanced stage of embryogenesis. This developmental response can serve as a bioassay for the identification of the defective component in eggs of deep orange females.
黑腹果蝇深橙色雌性（dor/dor）所产的卵存在缺陷，会导致由这些卵发育而来的深橙色胚胎过早死亡。将深橙色雌性的卵巢移植到正常雌性宿主中，这些卵巢会继续产出有缺陷的卵，这表明dor突变直接影响卵巢功能。在深橙色雌性所产卵子发育而来的杂合胚胎（dor(+)/dor）中，父本dor(+)基因的存在能使约一半的胚胎正常发育。因此，dor突变导致的卵缺陷在受精后可通过dor(+)基因的作用得到修复。在发育的合胞体胚盘前期，将未受精的正常卵子的细胞质注入深橙色胚胎，也能修复深橙色卵的缺陷。约三分之一接受注射的深橙色胚胎能够继续发育到胚胎发生的晚期阶段。这种发育反应可作为一种生物测定法，用于鉴定深橙色雌性卵子中的缺陷成分。
