Rutberg L, Armentrout R W, Jonasson J
J Virol. 1972 May;9(5):732-7. doi: 10.1128/JVI.9.5.732-737.1972.
SPO2 and phi105 are temperate Bacillus subtilis bacteriophages which have been suggested to belong to a cluster of related bacteriophages. In the present work, we show that SPO2 does not complement any of the 11 essential genes known in phi105 and that the phages do not recombine. Deoxyribonucleic acid (DNA)-DNA hybridization shows less than 10% homology between SPO2 and phi105 DNA. DNA synthesis in phi105 shows a greater dependence on host functions than does SPO2 DNA synthesis. Growth of phi105 but not of SPO2 is inhibited by the uracil analogue 6-(p-hydroxyphenylazo)-uracil. Infection of a DNA polymerase-deficient strain of B. subtilis with SPO2 leads to an increase in DNA polymerase activity in crude extracts, whereas no such increase is found after infection of this strain with phi105. It is concluded that SPO2 and phi105 are unrelated bacteriophages.
SPO2和phi105是温和型枯草芽孢杆菌噬菌体，有人认为它们属于一组相关噬菌体。在本研究中，我们发现SPO2不能互补phi105中已知的11个必需基因中的任何一个，并且这两种噬菌体不会发生重组。脱氧核糖核酸（DNA）-DNA杂交显示SPO2和phi105 DNA之间的同源性低于10%。phi105中的DNA合成比SPO2的DNA合成对宿主功能有更大的依赖性。尿嘧啶类似物6-（对羟基苯偶氮）-尿嘧啶可抑制phi105的生长，但不抑制SPO2的生长。用SPO2感染枯草芽孢杆菌的DNA聚合酶缺陷菌株会导致粗提物中DNA聚合酶活性增加，而用phi105感染该菌株后未发现这种增加。得出的结论是，SPO2和phi105是不相关的噬菌体。
