Zuniga M C, Durham D R, Welch R A
J Bacteriol. 1981 Sep;147(3):836-43. doi: 10.1128/jb.147.3.836-843.1981.
Pseudomonas putida PMD-1 dissimilates naphthalene (Nah), salicylate (Sal), and benzoate (Ben) via catechol which is metabolized through the meta (or alpha-keto acid) pathway. The ability to utilize salicylate but not naphthalene was transferred from P. putida PMD-1 to several Pseudomonas species. Agarose gel electrophoresis of deoxyribonucleic acid (DNA) from PMD-1 and Sal+ exconjugants indicated that a plasmid (pMWD-1) of 110 megadaltons is correlated with the Sal+ phenotype; restriction enzyme analysis of DNA from Sal+ exconjugants indicated that plasmid pMWD-1 was transmitted intact. Enzyme analysis of Sal+ exconjugants demonstrated that the enzymes required to oxidize naphthalene to salicylate are absent, but salicylate hydroxylase and enzymes of the meta pathway are present. Thus, naphthalene conversion to salicylate requires chromosomal genes, whereas salicylate degradation is plasmid encoded. Comparison of restriction digests of plasmid pMWD-1 indicated that it differs considerably from the naphthalene and salicylate degradative plasmids previously described in P. putida.
恶臭假单胞菌PMD - 1通过儿茶酚异化萘（Nah）、水杨酸盐（Sal）和苯甲酸盐（Ben），儿茶酚通过间位（或α - 酮酸）途径进行代谢。利用水杨酸盐而非萘的能力从恶臭假单胞菌PMD - 1转移到了几种假单胞菌属物种。对来自PMD - 1和Sal + 接合后体的脱氧核糖核酸（DNA）进行琼脂糖凝胶电泳表明，一个110兆道尔顿的质粒（pMWD - 1）与Sal + 表型相关；对Sal + 接合后体的DNA进行限制性酶切分析表明，质粒pMWD - 1完整地进行了传递。对Sal + 接合后体的酶分析表明，不存在将萘氧化为水杨酸盐所需的酶，但存在水杨酸盐羟化酶和间位途径的酶。因此，萘转化为水杨酸盐需要染色体基因，而水杨酸盐的降解由质粒编码。对质粒pMWD - 1的限制性酶切消化产物进行比较表明，它与先前在恶臭假单胞菌中描述的萘和水杨酸盐降解质粒有很大不同。
