Kuznetsov D I, Semenova L I
Vopr Pitan. 1979 May-Jun(3):56-66.
Two ways of calculating the content of individual fatty acids in the foods based on the data of gas liquid and thin-layer chromatography (through average values calculation) or only on those of the first (through addition of standard) are advisable. Most reliable in the last case is addition of trimargarine as a standard. The evaluation of the calculation errors is given. A variation of acidic-alkaline methanolysis of fat and esterification of free fatty acids in a closed system is proposed. By using this procedure the yield of methyl esters of fatty acids forms 90 +/- 7 per cent.
基于气液色谱和薄层色谱数据(通过计算平均值)或仅基于气液色谱数据(通过添加标准品)来计算食品中单个脂肪酸含量的两种方法是可取的。在后一种情况下,添加人造奶油作为标准品最为可靠。文中给出了计算误差的评估。本文提出了在封闭系统中脂肪的酸碱甲醇解及游离脂肪酸酯化的改进方法。采用该方法时,脂肪酸甲酯的产率为90±7%。