Strniste G F, Taylor W D
J Virol. 1972 Nov;10(5):1031-6. doi: 10.1128/JVI.10.5.1031-1036.1972.
Measurements of ribonucleic acid (RNA) synthesis using (14)C-uracil uptake in rifampin-sensitive and rifampin-resistant strains of Bacillus megatherium were carried out after infection of the bacteria with bacteriophage alpha. Phage development was inhibited in the former but not the latter strain, showing that alpha phage development is dependent on the bacterial host RNA polymerase. This dependence exists at all times during eclipse. RNA polymerase extracts showed the same in vitro rifampin sensitivity as the corresponding bacterial strains.
在用噬菌体α感染巨大芽孢杆菌的利福平敏感菌株和利福平抗性菌株后,利用(14)C -尿嘧啶摄取对核糖核酸(RNA)合成进行了测量。噬菌体发育在前一种菌株中受到抑制,而在后一种菌株中未受抑制,这表明α噬菌体发育依赖于细菌宿主RNA聚合酶。这种依赖性在隐蔽期的所有时间都存在。RNA聚合酶提取物在体外显示出与相应细菌菌株相同的利福平敏感性。