Milanesi G, Cassani G
J Virol. 1972 Aug;10(2):187-92. doi: 10.1128/JVI.10.2.187-192.1972.
The role of the host polymerase in Bacillus subtilis infected with phage SPP1 was studied in vivo with regard to production of phage-specific and host-specific ribonucleic acid (RNA) and to phage yield. Evidence is presented that the subunit(s) of B. subtilis RNA polymerase which is sensitive to rifampin and streptolydigin is necessary at all times during infection for phage production. The synthesis of phage RNA and the phage yield in strains resistant to either antibiotic were unaffected by the drug. Host RNA synthesis continued throughout infection; phage-specific RNA never accounted for more than 20% of pulse-labeled RNA at any time during infection.
在体内研究了宿主聚合酶在被噬菌体SPP1感染的枯草芽孢杆菌中的作用,涉及噬菌体特异性和宿主特异性核糖核酸(RNA)的产生以及噬菌体产量。有证据表明,在感染期间的所有时间,对利福平和平阳霉素敏感的枯草芽孢杆菌RNA聚合酶亚基对于噬菌体产生都是必需的。对这两种抗生素有抗性的菌株中噬菌体RNA的合成和噬菌体产量不受药物影响。宿主RNA合成在整个感染过程中持续进行;在感染期间的任何时候,噬菌体特异性RNA从未占脉冲标记RNA的20%以上。