Zazimko L A
Vopr Virusol. 1979 May-Jun(3):205-10.
The degree of affinity of influenza A/WSN/33 and A/Singapore/57 virus genomes was studied by the method of molecular hybridization which showed the presence in their genomes of 54--57% of analogous sequences. The electrophoretic analysis of RNA--RNA hybrids formed in hybridization of both homologous and heterologous virion and virus-induced RNAs of influenza viruses revealed significant differences in base sequences of the IV, V, and VIII fragments of influenza A/WSN/33 virus RNA and the corresponding fragments of influenza A/Singapore/57 virus RNA; the III and VII fragments of these strains have sites also differing in base sequences at the ends of molecules.
采用分子杂交方法研究了甲型流感病毒/WSN/33和甲型流感病毒/新加坡/57病毒基因组的亲和程度,结果表明它们的基因组中存在54%-57%的相似序列。对同源和异源流感病毒粒子及病毒诱导的RNA杂交形成的RNA-RNA杂种进行电泳分析,结果显示甲型流感病毒/WSN/33病毒RNA的IV、V和VIII片段与甲型流感病毒/新加坡/57病毒RNA的相应片段在碱基序列上存在显著差异;这些毒株的III和VII片段在分子末端也有碱基序列不同的位点。