Housset P, Nagy M, Schwencke J
J Gen Microbiol. 1975 Oct;90(2):260-4. doi: 10.1099/00221287-90-2-260.
A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.
本文描述了一种将粟酒裂殖酵母细胞高效转化为原生质体的新方法。在全细胞中测定的鸟嘌呤摄取的以下参数在原生质体中未发生变化：米氏常数（Km值）、对能量源的需求、对竞争性抑制剂的敏感性、最适pH值，以及在生长阶段观察到的摄取初始速度的典型变化。
