Loskutoff D J, Pène J J
J Virol. 1973 Jan;11(1):87-97. doi: 10.1128/JVI.11.1.87-97.1973.
The ribonucleic acid (RNA) specified by bacteriophage phi29 was isolated under conditions which minimized physical and enzymatic degradation, reduced aggregation, and enriched for completed molecules. This RNA was fractionated both by sedimentation through sucrose density gradients and electrophoresis through polyacrylamide gels to measure the size and relative amount of each component. Early RNA consisted of six components of molecular weight 0.75 x 10(6), 0.44 x 10(6), 0.37 x 10(6), 0.25 x 10(6), 0.09 x 10(6), and 0.04 x 10(6), accounting for 35% of the coding capacity of phi29 deoxyribonucleic acid (DNA). All of these components except the one at 0.44 x 10(6) were detected when infection occurred in the presence of chloramphenicol. Synthesis of the major early component (0.75 x 10(6)) ceased shortly after the onset of viral DNA synthesis. The other species of early RNA were synthesized throughout the latent period. Three additional components, 1.75 x 10(6), 0.93 x 10(6), and 0.07 x 10(6), appear at late times. The two large RNAs may be polycistronic messenger RNAs corresponding to the seven viral capsid proteins.
在将物理和酶促降解降至最低、减少聚集并富集完整分子的条件下，分离出了由噬菌体phi29指定的核糖核酸（RNA）。通过蔗糖密度梯度沉降和聚丙烯酰胺凝胶电泳对该RNA进行分级分离，以测量每个组分的大小和相对含量。早期RNA由分子量分别为0.75×10⁶、0.44×10⁶、0.37×10⁶、0.25×10⁶、0.09×10⁶和0.04×10⁶的六个组分组成，占phi29脱氧核糖核酸（DNA）编码能力的35%。当在氯霉素存在下发生感染时，除了分子量为0.44×10⁶的那个组分外，所有这些组分都能被检测到。主要早期组分（0.75×10⁶）的合成在病毒DNA合成开始后不久就停止了。其他早期RNA种类在整个潜伏期都有合成。另外三个组分，1.75×10⁶、0.93×10⁶和0.07×10⁶，在后期出现。这两种大RNA可能是与七种病毒衣壳蛋白相对应的多顺反子信使RNA。
