噬菌体phi29脱氧核糖核酸在6-（对羟基苯偶氮）-尿嘧啶处理的枯草芽孢杆菌中的选择性复制。
Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis.
作者信息
Schachtele C F, Reilly B E, De Sain C V, Whittington M O, Anderson D L
出版信息
J Virol. 1973 Jan;11(1):153-5. doi: 10.1128/JVI.11.1.153-155.1973.
Abstract
Phage phi29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of phi29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage phi29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.
摘要
噬菌体φ29脱氧核糖核酸（DNA）在枯草芽孢杆菌宿主细胞中半保留DNA合成被6-（对羟基苯偶氮）尿嘧啶（HPUra）阻断的条件下进行复制。φ29 DNA复制的起始时间不受HPUra影响，并且在抑制剂存在的情况下产生了正常数量的有活力噬菌体。对噬菌体φ29条件致死突变体的研究证明了HPUra在检测病毒特异性DNA合成方面的有用性。
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