采用变色孔琼脂扩散技术对葡萄球菌核酸酶进行简便检测。
Convenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique.
作者信息
Lachica R V, Hoeprich P D, Franti C E
出版信息
Appl Microbiol. 1972 Dec;24(6):920-3. doi: 10.1128/am.24.6.920-923.1972.
Abstract
The metachromatic agar-diffusion (MAD) microslide technique was adapted for quantitative assay for staphylococcal thermonuclease in heterogeneous systems, such as milk and broth. When an enzyme-containing solution was placed in a well cut in the agar, a bright pink halo was obtained. The diameter of the pink zone of hydrolysis was related to time and temperature of incubation and to nuclease concentration. Concentrations of nuclease as low as 0.005 mug/ml and as high as 2.0 mug/ml were conveniently determined after 3 hr at 37 C.
摘要
变色琼脂扩散（MAD）微玻片技术被改编用于在诸如牛奶和肉汤等异质系统中对葡萄球菌耐热核酸酶进行定量测定。当将含酶溶液置于琼脂中切出的孔中时，会获得一个亮粉色晕圈。水解粉色区域的直径与孵育时间、温度以及核酸酶浓度有关。在37℃孵育3小时后，可方便地测定低至0.005微克/毫升和高达2.0微克/毫升的核酸酶浓度。
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