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葡萄球菌核酸酶的比浊法测定

Turbidimetric assay of staphylococcal nuclease.

作者信息

Erickson A, Deibel R H

出版信息

Appl Microbiol. 1973 Mar;25(3):337-41. doi: 10.1128/am.25.3.337-341.1973.

Abstract

A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and heat-labile nuclease activity.

摘要

开发了一种简化的比浊法来测定葡萄球菌核酸酶活性。该方法操作简便且对纳克量具有敏感性,提高了其在定量或定性评估该酶方面的应用。与平板接种法不同,比浊法能够区分热稳定和热不稳定的核酸酶活性。

相似文献

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Turbidimetric assay of staphylococcal nuclease.葡萄球菌核酸酶的比浊法测定
Appl Microbiol. 1973 Mar;25(3):337-41. doi: 10.1128/am.25.3.337-341.1973.
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Production and heat stability of staphylococcal nuclease.葡萄球菌核酸酶的产生及热稳定性
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引用本文的文献

6
Production and heat stability of staphylococcal nuclease.葡萄球菌核酸酶的产生及热稳定性
Appl Microbiol. 1973 Mar;25(3):332-6. doi: 10.1128/am.25.3.332-336.1973.

本文引用的文献

2
The turbidimetric determination of deoxyribonuclease activity.脱氧核糖核酸酶活性的比浊测定法
Arch Biochem Biophys. 1959 May;82(1):135-44. doi: 10.1016/0003-9861(59)90098-0.
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The microdetermination of ribonuclease.
Arch Biochem Biophys. 1958 Feb;73(2):384-90. doi: 10.1016/0003-9861(58)90283-2.

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