半固体和肉汤培养物中核酸酶活性的检测。
Detection of nuclease activity in semisolid and broth cultures.
作者信息
Lachica R V, Deibel R H
出版信息
Appl Microbiol. 1969 Aug;18(2):174-6. doi: 10.1128/am.18.2.174-176.1969.
Abstract
A method for the detection of deoxyribonuclease activity in semisolid agar cultures was investigated. When cultures were overlaid with an acridine orangedeoxyribonucleate-agar (ADA) mixture, incubated for 1 to 3 hr, and observed under ultraviolet light, clear halos developed around colonies that produced deoxyribonuclease. A variation of the method for use with broth cultures involved impregnation of filter-paper discs with a small portion of the culture and overlaying the discs with the ADA mixture. This alteration has the advantage that the test tube cultures are easily heat-treated prior to assay to determine the heat resistance of the enzyme.
摘要
研究了一种检测半固体琼脂培养物中脱氧核糖核酸酶活性的方法。当在培养物上覆盖吖啶橙 - 脱氧核糖核酸 - 琼脂（ADA）混合物，孵育1至3小时，然后在紫外线下观察时，产生脱氧核糖核酸酶的菌落周围会形成清晰的晕圈。该方法用于肉汤培养物的一种变体是用一小部分培养物浸渍滤纸圆盘，然后在圆盘上覆盖ADA混合物。这种改变的优点是在测定前易于对试管培养物进行热处理，以确定酶的耐热性。
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本文引用的文献
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Deoxyribonuclease activity of micrococci from clinical sources.临床来源微球菌的脱氧核糖核酸酶活性
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