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枯草芽孢杆菌168转化中不连锁标记的聚合与基因定位

Congression of unlinked markers and genetic mapping in the transformation of Bacillus subtilis 168.

作者信息

Erickson R J, Copeland J C

出版信息

Genetics. 1973 Jan;73(1):13-21. doi: 10.1093/genetics/73.1.13.

Abstract

A thorough examination of cotransformation of two unlinked genetic markers in Bacillus subtilis 168 shows that the two recombinational events do not occur randomly. The cotransformation frequency is dependent on the distance between the two markers as well as on the order in which they replicate in the competent cell. These results indicate that uptake and/or integration of DNA molecules bearing these genetic markers is enhanced at the time these markers replicate in the competent cell.

摘要

对枯草芽孢杆菌168中两个不连锁遗传标记的共转化进行的全面研究表明,这两个重组事件并非随机发生。共转化频率取决于两个标记之间的距离以及它们在感受态细胞中复制的顺序。这些结果表明,携带这些遗传标记的DNA分子的摄取和/或整合在这些标记于感受态细胞中复制时会增强。

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