枯草芽孢杆菌168中的双向染色体复制。
Bidirectional chromosome replication in Bacillus subtilis 168.
作者信息
Harford N
出版信息
J Bacteriol. 1975 Mar;121(3):835-47. doi: 10.1128/jb.121.3.835-847.1975.
Density transfer analysis of deoxyribonucleic acid from Bacillus subtilis 168 thy spores germinating in 5-bromouracil medium shows the order of replication of genetic markers to be: purA16, cysA14, sacA, ctrA, (narB, arol), dal, (hisA1, purB6), (tre-12, thr-5), (argA, aroG, argC4), (metC, leu-8, pheA), (ura-1, aroD), lys-1, (trpC, metB, ilvA, citB, citK, gltA). The precise order of transfer of markers within parentheses could not be determined in these experiments. Taken together with new PBS1 transduction data presented here and in the accompanying paper of J. Lepesant-Kejzlarová, J.-A. Lepesant, J. Walle, A. Billaut, and R. Dedonder (1975), the results can be resolved in terms of a symmetric, fully bidirectional mode of chromosome replication with a replication origin close to the purA16 marker and a terminus in the region of the gltA, citK loci, diametrically opposed to the origin. A new genetic map of the B. subtilis 168 chromosome is presented.
对在5-溴尿嘧啶培养基中萌发的枯草芽孢杆菌168 thy孢子的脱氧核糖核酸进行密度转移分析，结果表明遗传标记的复制顺序为：purA16、cysA14、sacA、ctrA、(narB, arol)、dal、(hisA1, purB6)、(tre-12, thr-5)、(argA, aroG, argC4)、(metC, leu-8, pheA)、(ura-1, aroD)、lys-1、(trpC, metB, ilvA, citB, citK, gltA)。在这些实验中无法确定括号内标记转移的精确顺序。结合本文以及J. Lepesant-Kejzlarová、J.-A. Lepesant、J. Walle、A. Billaut和R. Dedonder（1975年）的相关论文中给出的新的PBS1转导数据，这些结果可以用染色体复制的对称、完全双向模式来解释，复制起点靠近purA16标记，终点位于gltA、citK基因座区域，与起点相对。本文给出了枯草芽孢杆菌168染色体的新遗传图谱。
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