枯草芽孢杆菌的转化：L型菌或原生质体裂解物中脱氧核糖核酸的转化能力。
Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.
作者信息
Bettinger G E, Young F E
出版信息
J Bacteriol. 1975 Jun;122(3):987-93. doi: 10.1128/jb.122.3.987-993.1975.
Abstract
The transformation of Bacillus subtilis by homologous deoxyribonucleic acid (DNA) made available by gently lysing a stable L-form or protoplast suspension was 3 to 10-fold more efficient than DNA isolated by conventional procedures. This increased transformation was not influenced by digestion with pronase, trypsin, or ribonuclease. Preincubation of isolated DNA with L-form lysates did not increase the transformation efficiency above that achieved with untreated, isolated DNA. In addition to displaying a higher efficiency of transformation, the DNA found in these gently prepared lysates was also able to co-transform heretofore unlinked markers at frequencies in excess of those found by congression. Comparison of the frequency of multiple marker transformations to single marker events as a function of DNA dilution conclusively proves that these markers originated from the same continuous strand of DNA.
摘要
通过轻轻裂解稳定的L型菌或原生质体悬液获得的同源脱氧核糖核酸（DNA）对枯草芽孢杆菌的转化效率比通过传统方法分离的DNA高3至10倍。这种转化效率的提高不受链霉蛋白酶、胰蛋白酶或核糖核酸酶消化的影响。将分离的DNA与L型菌裂解物预温育，其转化效率并未高于未处理的分离DNA。除了具有更高的转化效率外，这些经温和制备的裂解物中发现的DNA还能够以超过通过染色体移动所发现的频率共转化此前未连锁的标记。将多标记转化与单标记事件的频率作为DNA稀释的函数进行比较，最终证明这些标记源自同一条连续的DNA链。
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