Gordon A M, Rowan R M, Brown T, Carson H G
J Clin Pathol. 1973 Jan;26(1):52-6. doi: 10.1136/jcp.26.1.52.
A modified nitroblue tetrazolium test (NBT) is described which is suitable for routine application in the haematology or bacteriology laboratory and which provides a rapid aid to the diagnosis of bacterial infection. Hitherto published methods have recommended the use of heparinized whole blood samples for the performance of the NBT test. However, we have demonstrated that the use of the sucrose polymer Ficoll permits the test to be carried out on buffy coats prepared from venous blood anticoagulated in sequestrene (EDTA). The mean percentage of NBT-positive neutrophils in 60 healthy control subjects was 6.1. The mean percentage of NBT-positive neutrophils in 56 patients with confirmed or presumed bacterial infection was 34.2. Scores within the normal range were observed in 13 patients in this group. Forty-eight individuals with viral infection yielded a mean percentage of NBT-positive neutrophils of 8.5. Six patients in this group gave scores above the upper limit of normal. Both pathological groups are described in detail and applications of this test are suggested.
本文描述了一种改良的硝基四氮唑蓝试验（NBT），该试验适用于血液学或细菌学实验室的常规应用，能为细菌感染的诊断提供快速辅助。此前发表的方法推荐使用肝素化全血样本进行NBT试验。然而，我们已证明使用蔗糖聚合物聚蔗糖能使该试验在由乙二胺四乙酸（EDTA）抗凝的静脉血制备的血沉棕黄层上进行。60名健康对照者中性粒细胞NBT阳性的平均百分比为6.1。56名确诊或疑似细菌感染患者中性粒细胞NBT阳性的平均百分比为34.2。该组中有13名患者的得分在正常范围内。48名病毒感染个体中性粒细胞NBT阳性的平均百分比为8.5。该组中有6名患者的得分高于正常上限。对这两个病理组进行了详细描述，并提出了该试验的应用建议。
