枯草芽孢杆菌的温度敏感型DNA合成突变体——附录：对称染色体复制的密度转移理论
Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication.
作者信息
White K, Sueoka N
出版信息
Genetics. 1973 Feb;73(2):185-214. doi: 10.1093/genetics/73.2.185.
Abstract
A simple method for characterizing temperature-sensitive DNA synthesis mutants is described. The method uses density transfer and transformation techniques and is based on expected theoretical behavior of the chromosome population. A direct proof of inhibition of initiation of DNA replication is provided. The mutant dna-1, showing quick inhibition of initiation, is further characterized and mapped. An independent method for mapping genetic markers close to the origin, based on their transfer behavior after inhibition of initiation, is presented.
摘要
本文描述了一种用于鉴定温度敏感型DNA合成突变体的简单方法。该方法采用密度转移和转化技术，基于染色体群体的预期理论行为。提供了DNA复制起始受抑制的直接证据。对表现出快速起始抑制的突变体dna-1进行了进一步的表征和定位。提出了一种基于遗传标记在起始抑制后的转移行为来定位靠近原点的遗传标记的独立方法。
相似文献
1
Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication.
Genetics. 1973 Feb;73(2):185-214. doi: 10.1093/genetics/73.2.185.
2
Characteristics of a Bacillus subtilis W23 mutant temperature sensitive for initiation of chromosome replication.
J Bacteriol. 1975 Jan;121(1):121-7. doi: 10.1128/jb.121.1.121-127.1975.
3
Temperature-sensitive mutants of Bacillus subtilis. I. Multiforked replication and sequential transfer of DNA by a temperature-sensitive mutant.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):206-13. doi: 10.1073/pnas.65.1.206.
4
Temperature-sensitive initiation of chromosome replication in a mutant of Bacillus subtilis.
J Bacteriol. 1973 May;114(2):474-84. doi: 10.1128/jb.114.2.474-484.1973.
5
Temperature-sensitive deoxyribonucleic acid replication in a dnaC mutant of Bacillus subtilis.
J Bacteriol. 1975 Jan;121(1):173-83. doi: 10.1128/jb.121.1.173-183.1975.
6
Membrane attachment of the chromosome in Bacillus subtilis mutants temperature-sensitive in DNA replication.
Nucleic Acids Res. 1974 Dec;1(12):1775-82. doi: 10.1093/nar/1.12.1775.
7
Bidirectional chromosome replication in Bacillus subtilis 168.
J Bacteriol. 1975 Mar;121(3):835-47. doi: 10.1128/jb.121.3.835-847.1975.
8
Asymmetric bidirectional replication of Bacillus subtilis chromosome.
Nat New Biol. 1973 Aug 15;244(137):200-3. doi: 10.1038/newbio244200a0.
9
Membrane enrichment of genetic markers close to the origin and terminus during the deoxyribonucleic acid replication cycle in Bacillus subtilis.
J Bacteriol. 1979 Sep;139(3):911-6. doi: 10.1128/jb.139.3.911-916.1979.
10
Control of deoxyribonucleic acid synthesis in a Bacillus subtilis mutant temperature sensitive for initiation of chromosome replication.
J Bacteriol. 1974 Feb;117(2):329-36. doi: 10.1128/jb.117.2.329-336.1974.
引用本文的文献
1
The replication terminator protein of the gram-positive bacterium Bacillus subtilis functions as a polar contrahelicase in gram-negative Escherichia coli.
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11143-7. doi: 10.1073/pnas.91.23.11143.
2
Replication through the terminus region of the Bacillus subtilis chromosome is not essential for the formation of a division septum that partitions the DNA.
J Bacteriol. 1995 Oct;177(19):5711-5. doi: 10.1128/jb.177.19.5711-5715.1995.
3
Highly specific labeling of the Bacillus subtilis chromosome terminus.
J Bacteriol. 1980 Aug;143(2):1036-8. doi: 10.1128/jb.143.2.1036-1038.1980.
4
The Bacillus subtilis chromosome.
Microbiol Rev. 1980 Mar;44(1):57-82. doi: 10.1128/mr.44.1.57-82.1980.
5
DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1980 May;77(5):2834-8. doi: 10.1073/pnas.77.5.2834.
6
Membrane association of a Staphylococcus aureus plasmid in Bacillus subtilis.
J Bacteriol. 1980 Apr;142(1):339-43. doi: 10.1128/jb.142.1.339-343.1980.
7
Site-specific in vitro binding of plasmid pUB110 to Bacillus subtilis membrane fraction.
J Bacteriol. 1983 Jun;154(3):1184-94. doi: 10.1128/jb.154.3.1184-1194.1983.
8
Specific in vitro binding of a plasmid to a membrane fraction of Bacillus subtilis.
Proc Natl Acad Sci U S A. 1983 Jan;80(2):574-8. doi: 10.1073/pnas.80.2.574.
9
Membrane attachment of the chromosome in Bacillus subtilis mutants temperature-sensitive in DNA replication.
Nucleic Acids Res. 1974 Dec;1(12):1775-82. doi: 10.1093/nar/1.12.1775.
10
Control of deoxyribonucleic acid synthesis in a Bacillus subtilis mutant temperature sensitive for initiation of chromosome replication.
J Bacteriol. 1974 Feb;117(2):329-36. doi: 10.1128/jb.117.2.329-336.1974.
本文引用的文献
1
THE REPLICATION OF DNA IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671-82. doi: 10.1073/pnas.44.7.671.
2
A METHOD FOR SELECTIVE ENRICHMENT OF MUTANTS BASED ON THE HIGH UV SENSITIVITY OF DNA CONTAINING 5-BROMOURACIL.
Biochem Biophys Res Commun. 1965 Jun 18;20:93-7.
3
Development of competence in the Bacillus subtilis transformation system.
J Bacteriol. 1967 Sep;94(3):562-70. doi: 10.1128/jb.94.3.562-570.1967.
4
On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
J Mol Biol. 1970 Nov 14;53(3):369-87. doi: 10.1016/0022-2836(70)90072-0.
5
Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-.
Cold Spring Harb Symp Quant Biol. 1968;33:799-808. doi: 10.1101/sqb.1968.033.01.092.
6
On the origin and direction of replication of the Escherichia coli K12 chromosome.
J Mol Biol. 1968 Mar 28;32(3):611-29. doi: 10.1016/0022-2836(68)90346-x.
7
Replication of the escherichia coli K12 chromosome.
Proc Natl Acad Sci U S A. 1967 Nov;58(5):1911-8. doi: 10.1073/pnas.58.5.1911.
8
Origin and sequence of chromosome replication in Escherichia coli.
J Mol Biol. 1972 Oct 14;70(3):549-66. doi: 10.1016/0022-2836(72)90559-1.
Zotero 插件