枯草芽孢杆菌的温度敏感型DNA合成突变体——附录：对称染色体复制的密度转移理论
Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication.
作者信息
White K, Sueoka N
出版信息
Genetics. 1973 Feb;73(2):185-214. doi: 10.1093/genetics/73.2.185.
Abstract
A simple method for characterizing temperature-sensitive DNA synthesis mutants is described. The method uses density transfer and transformation techniques and is based on expected theoretical behavior of the chromosome population. A direct proof of inhibition of initiation of DNA replication is provided. The mutant dna-1, showing quick inhibition of initiation, is further characterized and mapped. An independent method for mapping genetic markers close to the origin, based on their transfer behavior after inhibition of initiation, is presented.
摘要
本文描述了一种用于鉴定温度敏感型DNA合成突变体的简单方法。该方法采用密度转移和转化技术，基于染色体群体的预期理论行为。提供了DNA复制起始受抑制的直接证据。对表现出快速起始抑制的突变体dna-1进行了进一步的表征和定位。提出了一种基于遗传标记在起始抑制后的转移行为来定位靠近原点的遗传标记的独立方法。
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