Mukai N, Kobayashi S
Am J Pathol. 1972 Nov;69(2):331-48.
A single intraperitoneal inoculation of 0.01 ml of virus fluid, 10(3.5) to 10(4.5) TCID(50) HeLa cells/0.1 ml, was made in 12 newborn hamsters. From 28 to 96 days after the virus inoculation, all 12 hamsters developed multiple, multicentric tumors in the peritoneal cavity. Histopathologic observations on these abdominal tumors strongly suggest that the so-called adenovirus-typical undifferentiated neoplasm produced by intraperitoneal inoculation may originate in part from a neuronal precursor cell group destined for the myenteric plexus. A histochemical study has indicated no direct evidence that the intracytoplasmic basophilic corpuscle is comparable to that of the enterochromaffin pigment. A cytologic perusal under electron microscopy has merely suggested that the poorly differentiated tumor cells closely resemble those described in undifferentiated neurogenic tumors. The mechanism of selective susceptibility of certain neuronal precursor cells to adenovirus remains unanswered.
给12只新生仓鼠进行腹腔内单次接种0.01 ml病毒液，病毒液含10(3.5)至10(4.5) TCID(50) HeLa细胞/0.1 ml。在接种病毒后28至96天，所有12只仓鼠腹腔内均出现多个多中心肿瘤。对这些腹部肿瘤的组织病理学观察强烈提示，腹腔内接种产生的所谓腺病毒典型未分化肿瘤可能部分起源于注定要进入肌间神经丛的神经元前体细胞群。一项组织化学研究未发现直接证据表明胞质嗜碱性小体与肠嗜铬色素的小体相当。电子显微镜下的细胞学检查仅提示低分化肿瘤细胞与未分化神经源性肿瘤中描述的细胞非常相似。某些神经元前体细胞对腺病毒选择性易感性的机制仍未得到解答。
