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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Labow R S, Layne D S

Biochem J. 1972 Jul;128(3):491-7. doi: 10.1042/bj1280491.

Rabbit liver microsomal fractions in vitro effected the transfer of glucuronic acid from UDP-glucuronic acid to biochanin A, formononetin, daidzein, genistein and equol. Only monoglucuronides were formed. 2. The same isoflavones were converted into monoglucosides when UDP-[6-(3)H]glucose was substituted for UDP-glucuronic acid in the incubation medium in vitro. The glucosides were formed in much lesser yield than were the glucuronides. 3. The glucoside of genistein was identified as genistin (genistein 7-glucoside) by Sephadex chromatography and reverse isotope dilution. 4. The specificity of the glucuronyl- and glucosyl-transfer mechanisms was compared for a series of steroids and other phenols in addition to the isoflavones. It was concluded that separate transferases were responsible for the formation of the two types of glycosides.

兔肝微粒体组分在体外可使葡萄糖醛酸从尿苷二磷酸葡萄糖醛酸转移至鹰嘴豆芽素A、芒柄花黄素、大豆苷元、染料木黄酮和雌马酚。仅形成了单葡萄糖醛酸苷。2. 当在体外孵育培养基中用尿苷二磷酸-[6-(3)H]葡萄糖替代尿苷二磷酸葡萄糖醛酸时，相同的异黄酮被转化为单葡萄糖苷。葡萄糖苷的生成量远低于葡萄糖醛酸苷。3. 通过葡聚糖凝胶色谱法和反向同位素稀释法，染料木黄酮的葡萄糖苷被鉴定为染料木苷（染料木黄酮7-葡萄糖苷）。4. 除异黄酮外，还对一系列甾体和其他酚类物质的葡萄糖醛酸基转移和葡萄糖基转移机制的特异性进行了比较。得出的结论是，两种类型糖苷的形成由不同的转移酶负责。

Labow R S, Layne D S

Biochem J. 1972 Jul;128(3):491-7. doi: 10.1042/bj1280491.

Rabbit liver microsomal fractions in vitro effected the transfer of glucuronic acid from UDP-glucuronic acid to biochanin A, formononetin, daidzein, genistein and equol. Only monoglucuronides were formed. 2. The same isoflavones were converted into monoglucosides when UDP-[6-(3)H]glucose was substituted for UDP-glucuronic acid in the incubation medium in vitro. The glucosides were formed in much lesser yield than were the glucuronides. 3. The glucoside of genistein was identified as genistin (genistein 7-glucoside) by Sephadex chromatography and reverse isotope dilution. 4. The specificity of the glucuronyl- and glucosyl-transfer mechanisms was compared for a series of steroids and other phenols in addition to the isoflavones. It was concluded that separate transferases were responsible for the formation of the two types of glycosides.

兔肝微粒体组分在体外可使葡萄糖醛酸从尿苷二磷酸葡萄糖醛酸转移至鹰嘴豆芽素A、芒柄花黄素、大豆苷元、染料木黄酮和雌马酚。仅形成了单葡萄糖醛酸苷。2. 当在体外孵育培养基中用尿苷二磷酸-[6-(3)H]葡萄糖替代尿苷二磷酸葡萄糖醛酸时，相同的异黄酮被转化为单葡萄糖苷。葡萄糖苷的生成量远低于葡萄糖醛酸苷。3. 通过葡聚糖凝胶色谱法和反向同位素稀释法，染料木黄酮的葡萄糖苷被鉴定为染料木苷（染料木黄酮7-葡萄糖苷）。4. 除异黄酮外，还对一系列甾体和其他酚类物质的葡萄糖醛酸基转移和葡萄糖基转移机制的特异性进行了比较。得出的结论是，两种类型糖苷的形成由不同的转移酶负责。