Linares L I, Friedman L
Infect Immun. 1972 May;5(5):681-7. doi: 10.1128/iai.5.5.681-687.1972.
Virulence and infectivity of nine strains of Paracoccidioides brasiliensis were investigated in groups of mice which were inoculated intranasally or intravenously, and some of each were treated with corticosteroids. Fatal infections were not often seen among untreated mice, but mortality usually occurred when corticosteroids were given, regardless of the route of fungus inoculation. Prior treatment did not uniformly increase the incidence of infection, however; only in the case of intranasally inoculated mice was this effect seen. Most strains appeared to be more virulent when administered intravenously, with the exception of a single strain which, under the influence of corticosteroids, repeatedly displayed greatest virulence when given intranasally. All animals that died early in the course of the disease, irrespective of route of inoculation, always had acute pulmonary lesions and usually no other organ was involved. Animals which died later or were sacrificed always had chronic lung lesions. Whether or not chronically diseased animals had additional organ involvement correlated with how the organisms were administered; intravenously inoculated animals usually had extrapulmonary as well as pulmonary lesions, but lesions of those inoculated intranasally were almost exclusively pulmonary. Corticosteroids did not alter the histologic characteristics of either the acute or the chronic type of lesion, but the lesions of treated animals were usually more extensive. Most of the survivors appeared healthy even when infection was extensive.
在经鼻内或静脉内接种的小鼠组中研究了九株巴西副球孢子菌的毒力和传染性，每组中的部分小鼠用皮质类固醇进行治疗。在未治疗的小鼠中不常出现致命感染，但无论真菌接种途径如何，给予皮质类固醇时通常会发生死亡。然而，预先治疗并未一致地增加感染发生率；仅在经鼻内接种的小鼠中观察到这种效果。大多数菌株静脉给药时似乎毒力更强，但有一个菌株除外，在皮质类固醇的影响下，经鼻内给药时反复表现出最大毒力。在疾病过程早期死亡的所有动物，无论接种途径如何，总是有急性肺部病变且通常无其他器官受累。后期死亡或被处死的动物总是有慢性肺部病变。慢性患病动物是否有其他器官受累与接种方式有关；静脉内接种的动物通常有肺外以及肺部病变，但经鼻内接种的动物病变几乎仅在肺部。皮质类固醇不会改变急性或慢性病变类型的组织学特征，但治疗动物的病变通常更广泛。即使感染广泛，大多数幸存者看起来也是健康的。
