Becker C G, Levi R, Zavecz J
Am J Pathol. 1979 Jul;96(1):249-55.
Neonatal California White rabbits were sensitized with a glycoprotein purified from cured Virginia Bright tobacco leaves. Their serums, but not serums from normal rabbits, were demonstrated by passive cutaneous anaphylaxis technique to contain heat-labile, homocytophilic antibodies to this antigen and to similar material purified from cigarette smoke condensate. Serums containing IgE antibodies to tobacco antigen would not be demonstrated to contain hemagglutinating antibodies to this antigen. These experiments demonstrate that antigen capable of triggering specific IgE-mediated release of inflammatory mediators is present in cigarette smoke. It can be hypothesized that IgE-mediated responses to antigen in cigarette smoke are causally related to the development of vascular injury and of myocardial arrhythmia in hypersensitive smokers.
新生的加利福尼亚白兔用从弗吉尼亚烤烟烟叶中纯化的一种糖蛋白进行致敏。通过被动皮肤过敏反应技术证明，它们的血清中含有对该抗原以及从香烟烟雾浓缩物中纯化的类似物质的热不稳定、亲同种细胞的抗体，而正常兔子的血清中则没有。含有针对烟草抗原的IgE抗体的血清不会被证明含有针对该抗原的血凝抗体。这些实验表明，香烟烟雾中存在能够引发特异性IgE介导的炎症介质释放的抗原。可以推测，对香烟烟雾中抗原的IgE介导反应与过敏吸烟者血管损伤和心律失常的发生有因果关系。
