Sofer W H, Hatkoff M A
Genetics. 1972 Nov;72(3):545-9. doi: 10.1093/genetics/72.3.545.
We describe a selection procedure which utilizes the vapor from an unsaturated alcohol, 1-pentene-3-ol, for the detection and isolation of mutant flies with little or no alcohol dehydrogenase activity. ADH-negative flies are unaffected by exposure to the unsaturated alcohol, but ADH positives (wild-types) die after short exposure. The technique can be used to select rare ADH-negative individuals from large populations of wild-type flies.
我们描述了一种筛选程序，该程序利用不饱和醇1-戊烯-3-醇的蒸汽来检测和分离酒精脱氢酶活性低或无活性的突变果蝇。暴露于不饱和醇中，酒精脱氢酶阴性的果蝇不受影响，但酒精脱氢酶阳性（野生型）果蝇在短时间暴露后死亡。该技术可用于从大量野生型果蝇群体中筛选罕见的酒精脱氢酶阴性个体。
