果蝇中乙醇脱氢酶阴性突变体的化学筛选
Chemical selection of alcohol dehydrogenase negative mutants in drosophila.
作者信息
Sofer W H, Hatkoff M A
出版信息
Genetics. 1972 Nov;72(3):545-9. doi: 10.1093/genetics/72.3.545.
Abstract
We describe a selection procedure which utilizes the vapor from an unsaturated alcohol, 1-pentene-3-ol, for the detection and isolation of mutant flies with little or no alcohol dehydrogenase activity. ADH-negative flies are unaffected by exposure to the unsaturated alcohol, but ADH positives (wild-types) die after short exposure. The technique can be used to select rare ADH-negative individuals from large populations of wild-type flies.
摘要
我们描述了一种筛选程序，该程序利用不饱和醇1-戊烯-3-醇的蒸汽来检测和分离酒精脱氢酶活性低或无活性的突变果蝇。暴露于不饱和醇中，酒精脱氢酶阴性的果蝇不受影响，但酒精脱氢酶阳性（野生型）果蝇在短时间暴露后死亡。该技术可用于从大量野生型果蝇群体中筛选罕见的酒精脱氢酶阴性个体。
相似文献
1
Chemical selection of alcohol dehydrogenase negative mutants in drosophila.
Genetics. 1972 Nov;72(3):545-9. doi: 10.1093/genetics/72.3.545.
2
Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.
Genetics. 1975 Jan;79(1):73-83. doi: 10.1093/genetics/79.1.73.
3
Tolerance to 1-pentene-3-ol and to 1-pentene-3-one in relation to alcohol dehydrogenase (ADH) and aldo keto reductase (AKR) activities in Drosophila melanogaster.
Biochem Genet. 1990 Oct;28(9-10):513-22. doi: 10.1007/BF00554379.
4
Participation of Drosophila melanogaster alcohol dehydrogenase (ADH) in the detoxification of 1-pentene-3-ol and 1-pentene-3-one.
Heredity (Edinb). 1988 Aug;61 ( Pt 1):85-91. doi: 10.1038/hdy.1988.93.
5
Chemical selection of mutants that affect ADH activity in Drosophila. III. Effects of ethanol.
Biochem Genet. 1976 Feb;14(1-2):127-35. doi: 10.1007/BF00484879.
6
Synthesis and degradation of alcohol dehydrogenase in wild-type and Adh-null activity mutants of Drosophila melanogaster.
Biochem Genet. 1982 Apr;20(3-4):297-313. doi: 10.1007/BF00484426.
7
Alcohol dehydrogenase-negative mutants in Drosophila: defects at the structural locus?
Genetics. 1976 May;83(1):125-36. doi: 10.1093/genetics/83.1.125.
8
Induction of suppressed ADH activity in Drosophila pachea exposed to different alcohols.
Biochem Genet. 2003 Dec;41(11-12):413-26. doi: 10.1023/b:bigi.0000007776.65086.74.
9
Comparison of tryptic peptide profiles of alcohol dehydrogenase from Drosophila melanogaster at different ages: a rapid procedure using high performance liquid chromatography.
J Biochem Biophys Methods. 1984 Dec;10(3-4):153-62. doi: 10.1016/0165-022x(84)90035-6.
10
Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.
Cell. 1983 Aug;34(1):59-73. doi: 10.1016/0092-8674(83)90136-8.
引用本文的文献
1
Selection affecting enzyme polymorphisms in laboratory populations of Drosophila melanogaster.
Oecologia. 1979 Dec;43(3):341-354. doi: 10.1007/BF00344960.
2
Arsenate as a potential negative selection agent for deficiency variants in cultured plant cells.
Planta. 1979 Jan;146(2):155-60. doi: 10.1007/BF00388226.
3
Genetic sexing in the mediterranean fruit fly, Ceratitis capitata, using the alcohol dehydrogenase locus.
Theor Appl Genet. 1986 Jul;72(4):455-7. doi: 10.1007/BF00289526.
4
A pollen selection system for alcohol-dehydrogenase-negative mutants in plants.
Genetics. 1976 May;83(1):63-5. doi: 10.1093/genetics/83.1.63.
5
A genetic and molecular analysis of the 46C chromosomal region surrounding the FMRFamide neuropeptide gene in Drosophila melanogaster.
Genetics. 1994 May;137(1):121-37. doi: 10.1093/genetics/137.1.121.
6
In vitro suppression of a nonsense mutant of Drosophila melanogaster.
Nucleic Acids Res. 1982 Nov 25;10(22):7145-52. doi: 10.1093/nar/10.22.7145.
7
The genetics of a small autosomal region of Drosophila melanogaster, including the structural gene for alcohol dehydrogenase. V. Characterization of X-ray-induced Adh null mutations.
Genetics. 1982 Nov;102(3):421-35. doi: 10.1093/genetics/102.3.421.
8
Genetic and cytogenetic studies of malic enzyme in Drosophila melanogaster.
Biochem Genet. 1981 Jun;19(5-6):525-34. doi: 10.1007/BF00484624.
9
Effect of environmental alcohol on in vivo properties of Drosophila alcohol dehydrogenase.
Biochem Genet. 1981 Apr;19(3-4):421-30. doi: 10.1007/BF00504285.
10
Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone.
Nucleic Acids Res. 1980 Dec 11;8(23):5649-67. doi: 10.1093/nar/8.23.5649.
本文引用的文献
1
Alcohol dehydrogenases: a polymorphism in Drosophila melanogaster.
J Exp Zool. 1965 Nov;160(2):147-54. doi: 10.1002/jez.1401600202.
2
Complete and mosaic visible mutations produced by ethyl methanesulphonate in Drosophila melanogaster.
Mutat Res. 1970 Sep;10(3):227-36. doi: 10.1016/0027-5107(70)90119-3.
3
Age-dependent changes in alcohol dehydrogenase in drosophila.
J Exp Zool. 1969 Jun;171(2):185-9. doi: 10.1002/jez.1401710206.
4
Alterations of genetics material for analysis of alcohol dehydrogenase isozymes of Drosophila melanogaster.
Ann N Y Acad Sci. 1968 Jun 14;151(1):441-55. doi: 10.1111/j.1749-6632.1968.tb11907.x.
Zotero 插件