果蝇中影响乙醇脱氢酶的突变体的化学筛选。II. 1-戊炔-3-醇的应用。
Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.
作者信息
O'Donnell J, Gerace L, Leister F, Sofer W
出版信息
Genetics. 1975 Jan;79(1):73-83. doi: 10.1093/genetics/79.1.73.
Abstract
We describe a procedure for the selection of alcohol dehyrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase positive. Using this procedure and formaldehyde to generate mutants, we were able to detect seven alcohol dehydrogenase negative mutants out of 350,000 individuals subjected to selection. At least five of the mutants contain small deletions that include the alcohol dehydrogenase locus.
摘要
我们描述了一种在果蝇中筛选乙醇脱氢酶阴性突变体的方法。该方法包括将卵和幼虫暴露于溶解在培养基中的低浓度1-戊炔-3-醇中。只有那些乙醇脱氢酶活性水平大幅降低的果蝇才能存活。此外，如果突变体的母亲是乙醇脱氢酶阳性，那么基因型为阴性的果蝇就会死亡。利用这一方法并使用甲醛来产生突变体，我们在接受筛选的350,000个个体中检测到了7个乙醇脱氢酶阴性突变体。这些突变体中至少有5个含有小的缺失，其中包括乙醇脱氢酶基因座。
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