Maddison S, Horrell R I
Brain Res Bull. 1979 Mar-Apr;4(2):259-66. doi: 10.1016/0361-9230(79)90290-9.
Single unit discharges in the ventromedial hypothalamic nucleus (VMH) and lateral hypothalamic area (LH) were extracellularly recorded in urethane anesthetised female rats, while various solutions were perfused through the stomach or duodenum via implanted polythene tubes. Perfusates, which were maintained at 38 degrees C, were 0.9% (w/v) NaCl, 2.5% NaCl, 5.25% glucose, 30% glucose, 5.0% casein hydrolysate, and liquid food. Only units which did not respond to somatosensory stimuli were tested. One hundred and twenty six units were recorded for periods of up to 3 hr, occasionally longer, in 57 animals. Of these, 74 were recorded during gastric perfusions and 52 during duodenal perfusions. Distension of the stomach elicited changes in firing rate in 16 LH and 8 VMH units. Both increases and decreases in firing rate in response to gastric distension were observed in both the LH and VMH. There was no evidence that nutrient or osmotic properties of the perfusates exerted any modifying influence on hypothalamic unit discharges. Distention of the duodenum by the perfusions elicited changes in firing rate in 12 LH and 6 VMH units. In this case all LH units decreased firing and all VMH units increased firing during distension of the lumen. In addition 2 VMH units appeared to increase their firing rate in association with glucose perfusions. The results are discussed in terms of the role of gastrointestinal feedback to the hypothalamus in food intake regulation.
在乌拉坦麻醉的雌性大鼠中，细胞外记录腹内侧下丘脑核（VMH）和外侧下丘脑区（LH）的单单位放电，同时通过植入的聚乙烯管将各种溶液经胃或十二指肠灌注。保持在38摄氏度的灌注液有0.9%（w/v）氯化钠、2.5%氯化钠、5.25%葡萄糖、30%葡萄糖、5.0%酪蛋白水解物和流质食物。仅测试对体感刺激无反应的单位。在57只动物中记录了126个单位，记录时间长达3小时，偶尔更长。其中，74个单位在胃灌注期间记录，52个单位在十二指肠灌注期间记录。胃扩张引起16个LH单位和8个VMH单位的放电频率变化。在LH和VMH中均观察到对胃扩张的放电频率增加和减少。没有证据表明灌注液的营养或渗透特性对下丘脑单位放电有任何调节作用。灌注引起的十二指肠扩张使12个LH单位和6个VMH单位的放电频率发生变化。在这种情况下，在管腔扩张期间，所有LH单位的放电减少，所有VMH单位的放电增加。此外，2个VMH单位在葡萄糖灌注时似乎增加了放电频率。根据胃肠道对下丘脑的反馈在食物摄入调节中的作用对结果进行了讨论。
