通过转化链球菌的生长曲线测定转化速率。
Determination of the rate of transformation from growth curves of transformed streptococci.
作者信息
Schlegel R, Slade H D
出版信息
J Bacteriol. 1972 Jul;111(1):199-202. doi: 10.1128/jb.111.1.199-202.1972.
Abstract
A rapid method of determining the rate of transformation of group H streptococci to streptomycin resistance has been developed. The new technique, which involves analysis of the growth response of transformed streptococci in liquid medium containing streptomycin, is independent of chain length fluctuations and is demonstrably more accurate than the standard plating method. The relatively short generation time of streptococci under these conditions permits transformation rates to be estimated in 9 to 14 hr depending on the number of transformants in the inocula as compared to 50 hr by the agar plate procedure.
摘要
已开发出一种快速测定H群链球菌向链霉素抗性转化速率的方法。这项新技术涉及分析转化后的链球菌在含链霉素的液体培养基中的生长反应，它不受链长波动的影响，并且明显比标准平板接种法更准确。在这些条件下，链球菌相对较短的代时使得根据接种物中转化体的数量，可在9至14小时内估算出转化速率，相比之下，琼脂平板法需要50小时。
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