鸡肝精氨酸酶的分子特征
Molecular characteristics of chicken liver arginase.
作者信息
Grazi E, Magri E
出版信息
Biochem J. 1972 Feb;126(3):667-74. doi: 10.1042/bj1260667.
Abstract
A purification procedure for the preparation of chicken liver arginase in a homogeneous form is presented. The enzyme hydrolyses both arginine and argininic acid. Kinetic analysis reveals that the enzyme binds arginine when the amino group is protonated or unprotonated; however, the unprotonated form seems to be hydrolysed more rapidly. The enzyme exchanges with the medium approx. 1.6Mn(2+) ions per molecule.
摘要
本文介绍了一种制备纯鸡肝精氨酸酶的纯化方法。该酶能水解精氨酸和精氨酸酸。动力学分析表明，当氨基质子化或未质子化时，该酶与精氨酸结合；然而，未质子化形式的水解似乎更快。该酶与介质交换约每分子1.6个锰离子。
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