L-丝氨酸O-硫酸盐的酶促降解。反应机制。
The enzymic degradation of L-serine O-sulphate. Mechanism of the reaction.
作者信息
Tudball N, Thomas P
出版信息
Biochem J. 1972 Jun;128(1):41-6. doi: 10.1042/bj1280041.
Abstract
- During the enzyme-catalysed degradation of l-serine O-sulphate no exchange occurs between the hydrogen atom attached to the alpha-carbon atom of the substrate and the tritiated water of the incubation medium. 2. The participation of an intermediate carbanion has been demonstrated in the degradation by performing the reaction in the presence of tetranitromethane. 3. Photo-oxidation of the enzyme in the presence of Rose Bengal led to a rapid inactivation of enzyme with the concomitant loss of four histidine residues/molecule. 4. Rose Bengal was also a non-competitive inhibitor of the enzyme.
摘要
- 在l-丝氨酸O-硫酸盐的酶催化降解过程中，底物α-碳原子上连接的氢原子与孵育介质中的氚水之间不发生交换。2. 通过在四硝基甲烷存在下进行反应，已证明中间体碳负离子参与了降解过程。3. 在孟加拉玫瑰红存在下对该酶进行光氧化导致酶迅速失活，同时每个分子损失四个组氨酸残基。4. 孟加拉玫瑰红也是该酶的非竞争性抑制剂。
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