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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Thmas J H, Fowler J A

Biochem J. 1969 Sep;114(2):299-305. doi: 10.1042/bj1140299.

Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the alphabeta-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the alphabeta-eliminating system remained constant in vitamin B(6)-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[(35)S]-sulphate in vivo appears to be normal in vitamin B(6)-deficient animals. 5. No incorporation of tritium into the alphabeta-eliminating system occurred after administration of tritiated pyridoxine to experimental animals.

曾尝试证明在大鼠肝脏系统中催化L-丝氨酸O-硫酸盐αβ消除反应的磷酸吡哆醛的存在。2. 旨在解析与蛋白质结合的辅因子的方法、对纯化酶系统的光谱检查以及对脱辅基（丙氨酸转氨酶）的重新激活尝试均未能证明磷酸吡哆醛的存在。3. 即使其他依赖磷酸吡哆醛的系统活性显著下降，αβ消除系统在维生素B6缺乏的动物体内活性仍保持恒定。4. 在维生素B6缺乏的动物体内，L-丝氨酸O-[(35)S]-硫酸盐的体内代谢似乎正常。5. 给实验动物施用氚标记的吡哆醇后，未发生氚掺入αβ消除系统的情况。

Thmas J H, Fowler J A

Biochem J. 1969 Sep;114(2):299-305. doi: 10.1042/bj1140299.

Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the alphabeta-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the alphabeta-eliminating system remained constant in vitamin B(6)-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[(35)S]-sulphate in vivo appears to be normal in vitamin B(6)-deficient animals. 5. No incorporation of tritium into the alphabeta-eliminating system occurred after administration of tritiated pyridoxine to experimental animals.

曾尝试证明在大鼠肝脏系统中催化L-丝氨酸O-硫酸盐αβ消除反应的磷酸吡哆醛的存在。2. 旨在解析与蛋白质结合的辅因子的方法、对纯化酶系统的光谱检查以及对脱辅基（丙氨酸转氨酶）的重新激活尝试均未能证明磷酸吡哆醛的存在。3. 即使其他依赖磷酸吡哆醛的系统活性显著下降，αβ消除系统在维生素B6缺乏的动物体内活性仍保持恒定。4. 在维生素B6缺乏的动物体内，L-丝氨酸O-[(35)S]-硫酸盐的体内代谢似乎正常。5. 给实验动物施用氚标记的吡哆醇后，未发生氚掺入αβ消除系统的情况。