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猪肝中L-丝氨酸O-硫酸盐降解系统的纯化及性质

The purification and properties of the L-serine O-sulphate degrading system of pig liver.

作者信息

Tudball N, Thomas P, Bailey-Wood R

出版信息

Biochem J. 1971 Mar;121(5):747-52. doi: 10.1042/bj1210747.

DOI:10.1042/bj1210747
PMID:5113489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1176662/
Abstract
  1. The enzyme system from pig liver responsible for the alphabeta-elimination of l-serine O-sulphate was purified 1000-fold. 2. Isoelectric focusing produced two enzymically active fractions with isoelectric points at pH5.6 and 5.9 respectively. 3. Osmometry and gel filtration showed both enzymes to possess molecular weights of approx. 54000. 4. The separate activities exhibited similar amino acid compositions.
摘要
  1. 负责从L-丝氨酸O-硫酸盐中进行αβ消除反应的猪肝酶系统被纯化了1000倍。2. 等电聚焦产生了两个具有酶活性的组分，其等电点分别为pH5.6和5.9。3. 渗透压测定和凝胶过滤表明这两种酶的分子量均约为54000。4. 各自的活性表现出相似的氨基酸组成。

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引用本文的文献

1
The enzymic degradation of L-serine O-sulphate by a specific system from pig liver. Studies on the mechanism of the reaction.猪肝特定系统对L-丝氨酸O-硫酸盐的酶促降解。反应机制研究。
Biochem J. 1972 Jan;126(1):187-91. doi: 10.1042/bj1260187.
2
The enzymic degradation of L-serine O-sulphate. Mechanism of the reaction.L-丝氨酸O-硫酸盐的酶促降解。反应机制。
Biochem J. 1972 Jun;128(1):41-6. doi: 10.1042/bj1280041.
3
Studies on the L-serine O-sulphate-degrading activity of pig liver.猪肝中L-丝氨酸O-硫酸酯降解活性的研究。
Biochem J. 1972 Jul;128(4):133P. doi: 10.1042/bj1280133pa.

本文引用的文献

1
The metabolism of potassium l-serine O[S]-sulphate in the rat.大鼠中L-丝氨酸O[S]-硫酸盐的代谢
Biochem J. 1962 Dec;85(3):456-60. doi: 10.1042/bj0850456.
2
Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme.L-丝氨酸O[S]-硫酸盐的酶促脱硫：该酶的细胞内定位
Biochem J. 1961 Oct;81(1):68-71. doi: 10.1042/bj0810068.
3
Endogenous sulphate acceptors in rat liver.大鼠肝脏中的内源性硫酸盐受体
Biochem J. 1960 Nov;77(2):294-304. doi: 10.1042/bj0770294.
4
The spectrophotometric determination of tyrosine and tryptophan in proteins.蛋白质中酪氨酸和色氨酸的分光光度测定法。
Biochem J. 1946;40(5-6):628-32. doi: 10.1042/bj0400628.
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
A kinetic and equilibrium analysis of the glutamic oxaloacetate transaminase mechanism.谷氨酸草酰乙酸转氨酶机制的动力学与平衡分析。
J Biol Chem. 1962 Jul;237:2109-22.
7
O-sulphate esters of L-serine, L-threonine and L-hydroxyproline.L-丝氨酸、L-苏氨酸和L-羟脯氨酸的O-硫酸酯
Biochem J. 1961 Apr;79(1):111-7. doi: 10.1042/bj0790111.
8
Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.天然pH梯度中两性电解质的等电分级分离、分析及特性鉴定。IV. 与肌红蛋白分离相关的分辨率的进一步研究。
Acta Chem Scand. 1966;20(3):820-34. doi: 10.3891/acta.chem.scand.20-0820.
9
A rat liver system that catalyses a pyridoxal phosphate-independent alpha beta-eliminto.一种催化不依赖磷酸吡哆醛的αβ消除反应的大鼠肝脏系统。
Biochem J. 1969 Sep;114(2):299-305. doi: 10.1042/bj1140299.
10
Studies on the enzymic degradation of L-serine O-sulphate by a rat liver preparation.大鼠肝脏制剂对L-丝氨酸O-硫酸酯的酶促降解研究。
Biochem J. 1967 Nov;105(2):467-72. doi: 10.1042/bj1050467.