Linder M C, Moor J R, Scott L E, Munro H N
Biochem J. 1972 Sep;129(2):455-62. doi: 10.1042/bj1290455.
The iron and ferritin content of rat liver and the species of ferritin present were examined from 4 days before to 3 weeks after birth. 1. Total iron and ferritin iron accumulated rapidly during the last days of gestation and from the second postnatal day underwent a steady depletion. 2. The amount of iron deposited before birth in the liver of each pup varied inversely with litter size and could be increased moderately by injection of iron into the mother before mating. 3. Intraperitoneal injection of iron 1 day after birth doubled the concentration of total iron, ferritin iron and ferritin protein in the liver over the next 24h, but at 3 weeks after birth it raised the very low concentrations of iron and ferritin severalfold. 4. As shown by electrophoretic migration, ferritin and dissociated ferritin subunits prepared from the livers of rats from 4 days before to 3 weeks after birth differed from those of adult liver ferritin and were indistinguishable from those of adult kidney and spleen ferritin. Treatment with iron at 3 weeks of age induced formation of a ferritin with electrophoretic properties resembling those of adult liver. It is concluded that iron given at this stage of development may activate the genetic cistron for adult liver ferritin.
对出生前4天至出生后3周的大鼠肝脏铁和铁蛋白含量以及存在的铁蛋白种类进行了检测。1. 总铁和铁蛋白铁在妊娠最后几天迅速积累，从出生后第二天开始稳定减少。2. 每只幼崽出生前肝脏中铁的沉积量与窝仔数成反比，在交配前给母体注射铁可适度增加铁的沉积量。3. 出生后1天腹腔注射铁，在接下来的24小时内肝脏中总铁、铁蛋白铁和铁蛋白蛋白的浓度增加了一倍，但在出生后3周时，它将极低的铁和铁蛋白浓度提高了几倍。4. 如电泳迁移所示，出生前4天至出生后3周大鼠肝脏制备的铁蛋白和解离的铁蛋白亚基与成年肝脏铁蛋白不同，与成年肾脏和脾脏铁蛋白无法区分。3周龄时用铁处理诱导形成了一种电泳性质类似于成年肝脏的铁蛋白。得出的结论是，在这个发育阶段给予铁可能会激活成年肝脏铁蛋白的遗传顺反子。
