Snyderman R, Pike M C, McCarley D, Lang L
Infect Immun. 1975 Mar;11(3):488-92. doi: 10.1128/iai.11.3.488-492.1975.
Delineation of the mechanisms of macrophage accumulation at local tissue sites will further our understanding of immunologically mediated host resistance to infectious and neoplastic diseases. Since mice are frequently used for the study of immune function, we developed a method for the quantification of mouse macrophage chemotaxis in vitro. By this method it was found that the fifth component of complement is necessary for the production of chemotactic activity in mouse serum by inflammatory agents such as endotoxin or aggregated gamma globulin. The majority of macrophage chemotactic activity produced by these agents in mouse serum can be attributed to a low-molecular-weight (ca. 15,000) chemotactic factor. The data suggest that this factor is the biologically active cleavage product of the fifth component of complement, C5a.
阐明巨噬细胞在局部组织部位积聚的机制，将有助于我们进一步理解免疫介导的宿主对感染性疾病和肿瘤性疾病的抵抗力。由于小鼠常用于免疫功能研究，我们开发了一种体外定量小鼠巨噬细胞趋化性的方法。通过该方法发现，补体的第五成分对于内毒素或聚集的γ球蛋白等炎症因子在小鼠血清中产生趋化活性是必需的。这些因子在小鼠血清中产生的大部分巨噬细胞趋化活性可归因于一种低分子量（约15,000）的趋化因子。数据表明，该因子是补体第五成分C5a的生物活性裂解产物。
