蛋白质向细胞核的迁移。I. 体内的蛙卵母细胞核积累显微注射的组蛋白，允许小蛋白质进入，并排斥大蛋白质。
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
作者信息
Bonner W M
出版信息
J Cell Biol. 1975 Feb;64(2):421-30. doi: 10.1083/jcb.64.2.421.
Abstract
A technique is presented which enables one to measure the extent to which a protein enters and accumulates in the nucleus of the frog oocyte. In this method, the protein, labeled with 125-I, is microinjected into the oocyte. After incubation, the oocyte is manually enucleated and the radioactivity in the nucleus and cytoplasm is determined. Using this technique, proteins lighter than 20,000 daltons were found to enter the nucleus and completely equilibrate between the nucleus and cytoplasm within 24 h. The entry of proteins heavier than 69,000 daltons was severely hindered. Histones and histone fractions entered as quickly as other small proteins, but, in contrast to these proteins, they accumulated in the nucleus to different extents, depending on the total amount of histone injected into the oocyte and the identity of the histone. Evidence is presented that histone fractions compete with each other for accumulation in the nucleus.
摘要
本文介绍了一种技术，该技术能够测量蛋白质进入青蛙卵母细胞核并在其中积累的程度。在这种方法中，用125-I标记的蛋白质被显微注射到卵母细胞中。孵育后，手动去除卵母细胞的细胞核，并测定细胞核和细胞质中的放射性。使用该技术发现，分子量小于20,000道尔顿的蛋白质会进入细胞核，并在24小时内在细胞核和细胞质之间完全达到平衡。分子量大于69,000道尔顿的蛋白质的进入受到严重阻碍。组蛋白和组蛋白组分与其他小蛋白质一样迅速进入，但与这些蛋白质不同的是，它们在细胞核中的积累程度不同，这取决于注射到卵母细胞中的组蛋白总量以及组蛋白的种类。有证据表明，组蛋白组分在细胞核积累方面相互竞争。
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Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.蛋白质向细胞核的迁移。I. 体内的蛙卵母细胞核积累显微注射的组蛋白，允许小蛋白质进入，并排斥大蛋白质。
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