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对选定的有丝分裂染色体进行激光微辐照后大鼠袋鼠(PTK2)细胞的克隆

Cloning of rat kangaroo (PTK2) cells following laser microirradiation of selected mitotic chromosomes.

作者信息

Basehoar G, Berns M W

出版信息

Science. 1973 Mar 30;179(4080):1333-4. doi: 10.1126/science.179.4080.1333.

Abstract

Mitotic chromosomes of rat kangaroo cells were irradiated with a green argon laser microbeam without prior dye sensitization. Deoxyribonucleic acid-negative chromosome paling was observed. The irradiated cells were isolated and cloned into viable populations.

摘要

用绿色氩激光微束对大鼠袋鼠细胞的有丝分裂染色体进行照射,无需事先进行染料敏化。观察到脱氧核糖核酸阴性的染色体变淡。将受照射的细胞分离并克隆成存活群体。

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