通过紫外激光微照射建立PtK2（长鼻袋狸）核仁缺陷亚系。
Establishment of nucleolar deficient sublines of PtK2 (Potorous tridactylis) by ultraviolet laser microirradiation.
作者信息
Liang H, Berns M W
出版信息
Exp Cell Res. 1983 Mar;144(1):234-40. doi: 10.1016/0014-4827(83)90461-5.
PMID:6840209
Abstract
One of the two nucleoli of tetraploid PtK2 WA cells in early prophase was irradiated with an ultraviolet (UV) laser microbeam. The daughter cells that maintained the nucleolar deficiency were isolated and cloned. Five nucleolar deficient sublines of PtK2 WA were established, thus providing an experimental system to study the ribosomal gene-nucleolar organizer complex.
摘要
在前期早期，用紫外（UV）激光微束照射四倍体PtK2 WA细胞的两个核仁之一。分离并克隆了维持核仁缺陷的子细胞。建立了五个PtK2 WA核仁缺陷亚系，从而提供了一个研究核糖体基因 - 核仁组织区复合体的实验系统。
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