Berns M W, Chong L K, Hammer-Wilson M, Miller K, Siemens A
Chromosoma. 1979 Jun 21;73(1):1-8. doi: 10.1007/BF00294839.
An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells.
在组织培养中，用紫外激光束聚焦于大鼠袋鼠细胞有丝分裂染色体的一个核仁组织区的亚微米点上。分离出子细胞并克隆成一个能存活的群体，该群体维持着定向的核仁缺陷。结论是激光可用于删除预先选定的遗传区域，并且这种基因删除在随后的子细胞中作为一种可遗传的缺陷得以维持。
