Haefelfinger P
J Chromatogr Sci. 1979 Jun;17(6):345-9. doi: 10.1093/chromsci/17.6.345.
A novel method for the determination of the antidepressant 3-(1-chloro-5-H-dibenzo [a,d] cycloheptene-5-ylidene)-N,N-dimethylpropylamine-N-oxide hydrochloride and its metabolites by use of high performance liquid chromatography was developed. The procedure is applicable to the assay of other similar drugs in biological samples. The method involves extraction of the unchanged drug and its metabolites from plasma, back-extraction into diluted phosphoric acid and re-extraction into an organic phase. Separation is performed on a silica gel column with an acidic mobile phase, containing sodium dodecyl sulfate as ion-pairing agent. The quantitation is carried out by UV detection. The procedure allows the determination of plasma levels down to about 5 ng/ml of the unchanged drug and its metabolites, respectively, when 1 ml of plasma is used. The plasma levels of two volunteers were determined after a single oral dose of the drug.
开发了一种利用高效液相色谱法测定抗抑郁药3-(1-氯-5-H-二苯并[a,d]环庚烯-5-亚基)-N,N-二甲基丙胺-N-氧化物盐酸盐及其代谢物的新方法。该方法适用于生物样品中其他类似药物的测定。该方法包括从血浆中提取未变化的药物及其代谢物，反萃取到稀释的磷酸中，然后再萃取到有机相中。在硅胶柱上用含有十二烷基硫酸钠作为离子对试剂的酸性流动相进行分离。通过紫外检测进行定量。当使用1ml血浆时，该方法分别能够测定血浆中低至约5ng/ml的未变化药物及其代谢物的水平。在两名志愿者单次口服该药物后测定了他们的血浆水平。
