Kuss B J, Nathmann M
Arzneimittelforschung. 1978;28(8):1301.
With regard to the increasing importance of determining plasma levels of antidepressant drugs a procedure has been worked out which makes it possible to analyze these substances in a simple and sensitive way. 5 ng )ca. 20 pmol) amitriptyline, nortriptyline, imipramine, desmethylimipramine, chlorimipramine, desmethylchlorimipramine, and protriptyline can be detected with a high-pressure liquid chromatograph and a UV-monitor without prior derivatisation. The extraction is made from 1 ml serum. Because of their retention time the substances can be identified. This fact allows simultaneous analyzation of all tricyclic antidepressants.
鉴于测定抗抑郁药物血浆水平的重要性日益增加，现已制定出一种方法，能够以简单且灵敏的方式分析这些物质。使用高压液相色谱仪和紫外监测器，无需预先衍生化，即可检测出5纳克（约20皮摩尔）的阿米替林、去甲替林、丙咪嗪、去甲丙咪嗪、氯米帕明、去甲氯米帕明和普罗替林。提取过程采用1毫升血清。根据这些物质的保留时间可进行鉴定。这一事实使得能够同时分析所有三环类抗抑郁药。
