氨氯地平对红细胞中钠钾通量的影响。

The effect of amiloride on sodium and potassium fluxes in red cells.

Aceves J, Cereijido M

J Physiol. 1973 Mar;229(3):709-18. doi: 10.1113/jphysiol.1973.sp010162.

Abstract

The effect of amiloride on the influx and efflux of (24)Na and (42)K in red cells was studied. The drug was added to the bathing Ringer or else incorporated in resealed ghosts.2. Amiloride does not inhibit the active or the passive (ouabain insensitive) extrusion of (24)Na.3. Amiloride inhibits the influx of (24)Na into red cells by 70%.4. Whether added to the inside or to the outside of the cells amiloride has no effect on the efflux of (42)K.5. Amiloride does not modify the uptake of (42)K from control Ringer. This uptake is strongly inhibited by the removal of Na. Amiloride has no effect on the extent of this inhibition.6. It is concluded that amiloride specifically inhibits the passive penetration of Na, and has no effect on the Na-K-pumping mechanism. However, at the concentration which inhibits 70% of the influx, amiloride fails to produce an observable effect on the ouabain-insensitive Na-efflux.7. On the basis that the information obtained could be extrapolated to other membranes, the effect of amiloride on epithelial membranes is discussed.

摘要

研究了氨氯吡咪对红细胞中（24）Na和（42）K流入及流出的影响。将该药物添加到浴用林格液中，或者掺入重封的血影中。
氨氯吡咪不抑制（24）Na的主动或被动（哇巴因不敏感）排出。
氨氯吡咪使（24）Na流入红细胞的量减少70%。
无论将氨氯吡咪添加到细胞内部还是外部，对（42）K的流出均无影响。
氨氯吡咪不改变从对照林格液中摄取（42）K的情况。去除Na会强烈抑制这种摄取。氨氯吡咪对这种抑制程度没有影响。
得出的结论是，氨氯吡咪特异性地抑制Na的被动渗透，对Na-K泵机制没有影响。然而，在抑制70%流入量的浓度下，氨氯吡咪对哇巴因不敏感的Na流出未产生可观察到的影响。
根据所获得的信息可外推至其他膜这一情况，讨论了氨氯吡咪对上皮膜的影响。