人血清抗胰蛋白酶活性的特征
Characteristics of the antitrypsin activity of human serum.
作者信息
Rowley P T, Oette D
出版信息
J Clin Pathol. 1973 Jan;26(1):48-51. doi: 10.1136/jcp.26.1.48.
The antitrypsin activity of human serum was studied as a function of pH, preincubation of serum with trypsin, and the concentrations of substrate, trypsin, and serum. Maximal activity was observed between pH 8.0 and 8.3. Activity was enhanced by preincubation of serum with trypsin, and reduced by high concentrations of trypsin and substrate. Percentage inhibition was a sigmoid function of log serum concentration. Theoretically, this relationship is expected of a reversible enzyme inhibitor; reversibility was demonstrated by dilution of enzyme serum mixtures. Practically, this relationship limits accurate antitryptic activity assay to the 25-75% inhibition range.
研究了人血清的抗胰蛋白酶活性与pH值、血清与胰蛋白酶的预孵育以及底物、胰蛋白酶和血清浓度之间的关系。在pH 8.0至8.3之间观察到最大活性。血清与胰蛋白酶预孵育可增强活性，而高浓度的胰蛋白酶和底物则会降低活性。抑制百分比是血清浓度对数的S形函数。从理论上讲，这种关系是可逆酶抑制剂所预期的；通过稀释酶血清混合物证明了其可逆性。实际上，这种关系将准确的抗胰蛋白酶活性测定限制在25%-75%的抑制范围内。
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